Name: Lei Lu
Email：lei_lu@tsinghua.edu.cn
Group website：https://lonelu.github.io
Research interest:
The long-term research objective is to develop de novo protein design methodologies, ultimately aiming to create practical artificial proteins for developing innovative therapeutic approaches. Our research group conducts both computational and experimental studies. The main research directions include:
1. De novo design of binding proteins for drugs, metals, glycans, and peptides
We focus on protein-molecule interactions, employing database search methods combined with novel protein design tools for designing binding proteins.
2. Computational method development and application for enzyme design
Designing enzymes to catalyze specific chemical reactions through new databases and developing deep learning approaches
3. Development of new proteomics technologies and algorithms
Creating novel proteomics technologies based on protein/enzyme design, apply to limited protease proteomics and proximity proteomics etc, ultimately enabling innovative drug screening and biomarker discovery.
Selected Publications：
(1)Chen, B.#; Guo, H.#; Yan, Z.; Lu, W.; Li, C.; Xu, S.; Zhang, Y.; Guo, H.; Sun, S.; Sun, X.; Zhao, S.; Shangguan, Q.; Chen, Y.; Lu, L.; Wu, Z.; Chen, Y.*; Qin, W.*. In vivo-compatible spatial multi-omics via hydrogen peroxide-independent APEX2 labeling. Nat. Chem. Biol. In press.
(2)Chen, YZ.#; Liu, ZY.#; Wang, YK.#; Lu, WJ.; Li, HY.; Wang, WJ.; Qiu, ZL.; Qiu, YJ.; Qing, H.; Xie, YX.; Liu, N.; Zhang, CG.*; Chen, Y.*; Qin, W.*. Multidimensional atlas of RNA-regulated proteins revealed by RNA-dependent thermal proteome profiling. BioRxiv, 2026.
(3)Chen, Z.#; Chen, YZ.#; Ding, HR.#; Huang, MD.#; Chen, WJ.; Fu, B.; Wang, MC.; Zhang, LQ.; Qin, W.*; Li, PL*. Mapping Core Components of Membrane-less Organelles in Living Cells by Phase-APEX2 Proximity Labeling. BioRxiv, 2025.
(4)Lu, WJ.#; Zhang, YL.#; Wang, P.; Ni, XR.; Zhuang, ST.*; Qin, W.*, Spatiotemporal profiling of modification-specific proteome secretion uncovers an itaconation-activated tyrosine kinase. Nat. Commun., 2025.
(5)Wang, WJ.#; Guo, HY.#; Yan, XS.#; Pan, XZ.; Wang, XF.; Rong, YM.; Bai, ZX.; Zhang, LW.; Wu, ZF.; Zhao, XY.; Huang, WR.; Qin, W.*; Chu, L.*, Silicon-rhodamine-enabled identification for near-infrared light controlled proximity labeling in vitro and in vivo.Nat Commun., 2025.
(6)Sun, XG.#; Zhang, YL.#; Lu, WJ.#; Guo, HY.#; He, GD.; Luo, SY.; Guo, HD.; Zhang, ZJ.; Wang, WJ.; Chu, L.; Liu, XY.; Qin, W.*, Precise and in vivo-compatible spatial proteomics via bioluminescence-triggered photocatalytic proximity labeling. ACS Cent. Sci., 2025.
(7)Zhang, ZJ.#; Wang, YK.#; Lu, WJ.; Wang, XF.; Guo, HY.; Pan, XZ.; Liu, ZY.; Wu, ZF.; Qin, W.*, Spatiotemporally resolved mapping of extracellular proteomes via in vivo-compatible TyroID. Nat. Commun., 2025.
(8)Sun, XG.#; Chen, Y.#; Yang, C.; Yang, S.; Lin, W.; Quan, BY.; Ding, Q.; Chen, X.*; Wang, C.*; Qin, W.*, Chemical recording of pump-specific drug efflux in living cells. Angew Chem Int Ed., 2024.
(9)Qin, W. #; Cheah, JS. #; Xu, C.; Messing, J.; Freibaum, BD.; Boeynaems, S.; Taylor, JP.; Udeshi, ND.; Carr, SA.; Ting, AY*. Dynamic mapping of proteome trafficking within and between living cells by TransitID. Cell, 2023.
